Nanobodies® (also kown as single-domain antibodies) (NANOBODY® and NANOBODIES® are registered trademarks of Ablynx NV) or heavy chain-only antibodies have become popular as reagents for biomedical research, as reagents for therapeutic use, and as reagents for diagnostics. The number of publications citing nanobodies is increasing each year with over 4,000 publications cited in PubMed to date. This is due, in large part, to their small size, stability, high affinity, high specificity, ease of manipulation, and ease of production.

Through resources provided by the National Institute of General Medical Sciences, our university based Nanobody Production Facility was established and is producing nanobodies (single-domain antibodies) in camelids on a fee for service basis. Our facility immunizes alpacas or llamas with your protein of interest, amplifies the produced pool of nanobody cDNAs by PCR and generates an immune library, which is then screened by panning against your protein.

Nanobodies to your target are purified and confirmed by E LISA. Deliverables include 0.5-1 mg of your purified single-domain antibody produced and purified from E. Coli, the single-domain antibody clone in an E. coli expression vector, and its sequence. To date we have successfully generated more than 100 nanobodies.

To produce a nanobody to your protein we require 1 to 1.5 mg of purified protein in PBS or HBS typically at a concentration of 1 mg/ ml or greater. We usually deliver your nanobody within 6 - 8 months, often sooner. We may identify more than one nanobody against a particular protein which can be purchased for a nominal cost.

To request a nanobody produced to your protein please fill out and return the request form, or contact Lou Hersh: