Basic Techniques and Lab Safety

"Making Solutions in the Laboratory"

The ability to successfully make solutions is a basic laboratory skill performed in virtually all biological and chemical experiments. 

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"Introduction to Light Microscopy"

The light microscope is an instrument used by researchers in many different fields to magnify specimens to as much as a thousand times their original size.

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"Decontamination for Lab Biosafety"

Decontamination is essential for laboratory biosafety, as the accumulation of microbial contamination in the laboratory can lead to the transmission of disease.

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"Safe Handling of Mineral Acids"

A mineral acid (or inorganic acid) is defined as a water-soluble acid derived from inorganic minerals by chemical reaction as opposed to organic acids.

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Protein Purification and Analysis

"Benchtop Immobilized Metal Affinity Chromatography, Reconstitution, and Assay of a Polyhistidine Tagged Metalloenzyme"

Benchtop immobilized metal affinity chromatography (IMAC), of polyhistidine tagged proteins is easily mastered by undergraduate students and has become the most widely used protein purification method in the modern literature. 

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"Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins"

Investigating multiple, endogenous post-translational modifications for a target protein can be extremely challenging. 

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"Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry"

A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry. 

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"Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web"

We demonstrate the usage of Bio3D-web for the interactive analysis of biomolecular structure data. 

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Molecular Biology

"Gel Purification of DNA"

Gel purification is used to recover DNA fragments after electrophoretic separation. 

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"DNA Ligation Reactions"

In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. 

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