JoVE Protocols These videos describe various techniques that may be of use the laboratory.
Basic Techniques and Lab Safety
"Introduction to Light Microscopy"
The light microscope is an instrument used by researchers in many different fields to magnify specimens to as much as a thousand times their original size.
"Decontamination for Lab Biosafety"
Decontamination is essential for laboratory biosafety, as the accumulation of microbial contamination in the laboratory can lead to the transmission of disease.
Protein Purification and Analysis
"Benchtop Immobilized Metal Affinity Chromatography, Reconstitution, and Assay of a Polyhistidine Tagged Metalloenzyme"
Benchtop immobilized metal affinity chromatography (IMAC), of polyhistidine tagged proteins is easily mastered by undergraduate students and has become the most widely used protein purification method in the modern literature.
"Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins"
Investigating multiple, endogenous post-translational modifications for a target protein can be extremely challenging.
"Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry"
A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry.