Gabrielle Keb, a third year Ph.D. student, recently published her innovative work building on a method developed in the Fields lab termed Fluorescence Reported Allelic Exchange Mutagenesis (FRAEM). Gabby engineered a CRE-lox system for generation of marker-less gene deletions in Chlamydia. Importantly, this elegant approach relieves polar effects on down-stream genes and enables a more definitive assessment of null phenotypes. The manuscript appears in Journal of Bacteriology (PMID: 30224436) and was chosen as a spotlight article. The commentary (PMID: 30297356), authored by Drs. Bastidas and Valdivia from Duke University, emphasizes how Gabby's new method will revolutionize chlamydial research.
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