Tribrid Fusion Orbitraps

The Thermo Tribrid Fusion is a Makarov-trap ("Orbitrap") Fourier-transform Mass Spectrometer (FTMS) capable of resolving power in excess of 350,000 @ m/z400, which was until recently achieved commercially only by ion-cyclotron resonance (ICR) MS. The Fusion instrument is capable of simultaneous, multi-mode ion processing and measurement via two collision/accumulator cells and two mass spectrometers (an Orbitrap and Ion Trap) with the ability to shuttle ions from either collision/accumulator cells to either MS. The strength of the Fusion is the ability to conduct very complex multistage tandem MS experiments, at great speed and extremely high resolution. This particular model is fitted with electron transfer dissociation (ETD) and internal lock-mass capabilities for ultra-stable mass reporting. The Fusion is typically combined with the Dionex ion chromatography systems or the Advion Nanomate direct nanoelectrospray sample/ion sources.

  •  A Thermo Tribrid Fusion FTMS Orbitrap is interfaced to chromatography, in particular the Dionex ion chromatography system, which can resolve a wide variety of negatively charged ions including phosphorylated intermediates and organic acids (4,5) as well as positively charged ions including polyamines. The IC system can also interface to the Agilent triple quadrupole ICP-MS (below).
  • The Lumos Orbitrap features up to 1,000,000 mass resolution and UV photodissociation and is also interfaced to an Advion Nanomate for direct infusion especially for lipid analysis (1) and amino acid analysis (3)  and tandem MS for metabolic subunit  isotopologue distribution analysis (6,7).


  1. Lane, A.N., Fan, T. W-M., Xie, X. Moseley, H.N. & Higashi, R.M. (2009) Stable isotope analysis of lipid biosynthesis by high resolution mass spectrometry and NMR. Anal. Chim. Acta. 651, 201-208
  2. Deng, P., Higashi, R.M., Lane, A.N., Bruntz, R.C., Sun, R.C., Raju, R.R., Nant, M.H., Qi, Z., Fan, T. W-M. (2018) Quantification and Identification of Carbonyls Using Chemoselective Tagging and Nanospray FT-MS. Analyst. 143, 311-322
  3. Yang, Y., Fan, W. W-M., Lane, A.N. & Higashi, R.M. (2017) Chloroformate Derivatization for Tracing the Fate of Amino Acids in Cells by Multiple Stable Isotope Resolved Metabolomics (mSIRM). Anal. Chim. Acta 976, 63-73
  4. Fan, T. W-M., Warmoes, M.O., Sun, Q., Song, H., Turchan-Cholewo, J., Martin, J.T., Mahan, A.L.,Higashi, R.M., Lane, A.N. (2016) Distinctly perturbed metabolic networks underlie differential tumor tissue damages induced by immune modulator b-glucan in a two-case ex vivo non-small cell lung cancer study. CSH Molec. Case Studies J. 2, a000893
  5. Q. Sun, T. W-M. Fan, A. N. Lane, R. M. Higashi (2019). Applications of Chromatography-Ultra High-resolution MS for Stable Isotope-Resolved Metabolomics (SIRM) Reconstruction of Metabolic Networks. TrAC 123, 115676
  6. 6. Sun, Q., Fan, T. W-M., Lane, A.N. & Higashi, R.M. (2021) Ion Chromatography-Ultra High-resolution MS1/MS2 Method for Stable Isotope-Resolved Metabolomics (SIRM) Reconstruction of Metabolic Networks. Anal. Chem. 93:2749-2757  
  7. 7. T. W-M. Fan, Q. Sun, R. M. Higashi (2022) Ultrahigh resolution MS1/MS2-based Reconstruction of Metabolic Networks in Mammalian Cells Reveals Changes for Selenite and Arsenic Action  J. Biol. Chem. 298: 102586  


The Agilent triple quad ICP MS is an ultra-high sensitivity mass spectrometer with high elemental ion selectivity across the periodic table. A Dionex ion chromatography module that resolves negatively charged analytes was installed Fall 2015. This enables speciation of compounds detected by element.

Laser Ablation

An Iridia laser ablation system (183 nm) has been interfaced to the ICP-MS. This enables spatial resolution of metals in thin tissue slices or other surfaces.