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Changhai Tian, PhD

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859-562-0290 (Office)
tch815@uky.edu
1095 V.A Drive 340 HSRB

Positions

  • Assistant Professor

College Unit(s)

Biography and Education

Education

Ph.D, Chinese of Academy of Sciences, P. R. China;

Post-doctoral training, University of Nebraska Medical Center, Omaha, NE USA

Research

Research My research interests are aimed at understanding the intra- and inter-organ communications by extracellular vesicles (EVs) during the progression of cardiovascular diseases and neurodegenerative disorders, and focused on the underlying mechanisms by which damaged organs communicate with remote organs during the disease process. In our studies, we will utilize different technologies involving cell biology, molecular biology, and physiology as well as stem cell biology to achieve our goals. In addition, several transgenic mouse models will also be used to support our research.

Selected Publications

  1. Tian C#, Gao L, Rudebush TL, Yu L and Zucker IH#. Extracellular vesicles regulate sympatho-excitation by Nrf2 in heart failure. Circulation Research. 2022;131 (8): 687–700. DOI: 10.1161/CIRCRESAHA.122.320916 PMID: 36098045 (#Corresponding authors)
  2. Tian C, Gao L and Zucker IH. Regulation of Nrf2 signaling pathway in heart failure: Role of extracellular vesicles and non-coding RNAs. Free Radic Biol Med. 2021, 167: 218-231. doi: 10.1016/j.freeradbiomed.2021.03.013. PMID: 33741451
  3. Gao L, Wang H, Tian C, and Zucker IH. Skeletal muscle Nrf2 contributes to systemic antioxidant defense via EV communication. Exerc Sport Sci Rev. 2021 Apr 22. doi: 10.1249/JES.0000000000000257. Online ahead of print. PMID: 33927165
  4. Chivero E, Liao K, Niu F, Tripathi A, Tian C, Buch S and Hu G. Engineered extracellular vesicles loaded with miR-124 attenuate cocaine-mediated activation of microglia. Front Cell Dev Biol. 2020 Jul 30; 8: 573. doi: 10.3389/fcell.2020.00573. PMID: 32850781.
  5. Tian C#, Hu G, Gao L, Hackfort BT, Zucker IH. Extracellular Vesicular MicroRNA-27a* Contributes to Cardiac Hypertrophy in Chronic Heart Failure. J Mol Cell Cardiol. 2020, 143: 120-131.doi: 10.1016/j.yjmcc.2020.04.032. PMID: 32370947 (#Corresponding author)
  6. Tian C#, Gao L, Zhang A, Hackfort B, Zucker IH. Therapeutic effects of Nrf2 activation by Bardoxolone methyl in Chronic Heart Failure. J Pharmacol Exp Ther. 2019 Dec; 371 (3): 642-651. doi: 10.1124/jpet.119.261792. PMID: 31601682 (#Corresponding author)
  7. Liao K, Niu F, Dagur RS, He M, Tian C, Hu G. Intranasal Delivery of lincRNA-Cox2 siRNA Loaded Extracellular Vesicles Decreases Lipopolysaccharide-Induced Microglial Proliferation in Mice. J Neuroimmune Pharmacol. 2019 Jul 20. doi: 10.1007/s11481-019-09864-z. PMID: 31325121.
  8. Ma Y, Wang K, Pan J, Fan Z, Tian C, Deng X, Ma K, Xia X, Huang Y, Zheng JC. Induced neural progenitor cells abundantly secrete extracellular vesicles and promote the proliferation of neural progenitors via extracellular signal-regulated kinase pathways. Neurobiol Dis. 2019 Apr; 124: 322-334. doi: 10.1016/j.nbd.2018.12.003. 
  9. He M, Zhang H, Li Y, Tian C, Tang B, Huang Y, Zheng J. Direct and selective lineage conversion of human fibroblasts to dopaminergic precursors. Neurosci Lett. 2019 Jan18; 699:16-23. doi: 10.1016/j.neulet.2019.01.033.
  10. Kangmu Ma, Xiaobei Deng, Xiaohuan Xia, Zhaohuan Fan, Xinrui Qi, Yongxiang Wang, Yuju Li, Yizhao Ma, Qiang Chen, Hui Peng, Jianqing Ding, Chunhong Li, Yunlong Huang#, Changhai Tian# and Jialin C. Zheng#. Direct conversion of mouse astrocytes into neural progenitor cells and specific lineages of neuron. Translational Neurodegeneration, 2018 Nov 5; 7: 29. doi: 10.1186/s40035-018-0132-x.  (#Co-Corresponding author)
  11. Guoku Hu, Ke Liao, Fang Niu, Lu Yang, Blake Dallon, Shannon Callen, Changhai Tian, Jiang Shu, Juan Cui, Zhiqiang Sun, Yuri Lyubchenko, Minhan Ka, Xian-Ming Chen, and Shilpa Buch. Astrocyte EV-induced lincRNA-Cox2 regulates microglial phagocytosis: Implications for morphine-mediated potentiation of neurodegeneration. Mol Ther Nucleic Acids. 2018 Dec 7; 13: 450-463. doi: 10.1016/j.omtn.2018.09.019.
  12. Tian C, Lie Gao, Matthew C. Zimmerman, Irving H. Zucker. Myocardial infarction-induced microRNA-enriched exosomes contribute to cardiac Nrf2 dysregulation in chronic heart failure. Am J Physiol Heart Circ Physiol. 2018 May 1; 314(5):H928-H939.
  13. Li Y, Peer J, Zhao R, Xu Y, Wu B, Wang Y, Tian C, Huang Y, Zheng J. Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration. Transl Neurodegener. 2017 Apr 20; 6:10. doi: 10.1186/s40035-017-0080-x.
  14. Tian C, Zucker IH. Therapeutic microRNA-based strategies in cardiovascular disease discriminate sex and age difference. J Physiol. 2016 Oct 15; 594(20):5731-5732. PMID:27739075
  15. Becker BK, Tian C, Zucker IH, Wang H. Influence of brain-derived neurotrophic factor-TrkB signaling in the NTS on baroreflex sensitivity in rats with chronic heart failure. J Physiol. 2016 May 6. doi: 10.1113/JP272318.
  16. Becker BK, Wang HJ, Tian C, Zucker IH. BDNF contributes to angiotensin II-mediated reductions in peak voltage-gated K+ current in cultured CATH. a cells. Physiol Rep. 2015 Nov; 3 (11). pii: e12598. doi: 10.14814/phy2.12598
  17. Changhai Tian#,*, Yuju Li#, Yunlong Huang#, Yongxiang Wang, Dapeng Chen, Jinxu Liu, Xiaobei Deng, Lijun Sun, Kristi Anderson, Xinrui Qi, Yulong Li, R. Lee Mosley, Xiangmei Chen, Jian Huang &Jialin C. Zheng*. Selective Generation of Dopaminergic Precursors from Mouse Fibroblasts by Direct Lineage Conversion. Scientific Reports. 2015, 5: 12622, DOI: 10.1038/srep12622. (*Co-Corresponding author)
  18. Lai S, Zhang M, Xu D, Zhang Y, Qiu L, Tian C*, Zheng JC*. Direct reprogramming of induced neural progenitors: a new promising strategy for AD treatment. Transl Neurodegener. 2015 Apr 18; 4: 7. (*Co-correspondence author).
  19. Changhai Tian*, Qiang Liu, Kangmu Ma, Yongxiang Wang, Qiang Chen, Randall Ambroz, David L. Klinkebiel, Yuju Li, Yunlong Huang, Jianqing Ding, Jie Wu, Jialin C. Zheng*. Characterization of Induced Neural Progenitors from Skin Fibroblasts by a Novel Combination of Defined Factors. Sci Rep. 2013 Feb 26; 3: 1345.  (*Co-correspondence Author). PMCID: PMC3581826.
  20. Changhai Tian and Jialin Zheng. Neural Progenitors by Direct Reprogramming: Strategies for the Treatment of Parkinson’s and Alzheimer’s Diseases. Science. 2013, 342 (6165 Suppl): 50–52. doi: 10.1126/science.1244943.
  21. Wang Y, Tian C*, Zheng J*. FoxO3a contributes to the reprogramming process and the differentiation of induced pluripotent stem cells. Stem Cells Dev. 2013 Nov 15; 22(22):2954-63. (*Co-correspondence author).
  22. Zhao X#, Tian C#, Puszyk WM, Ogunwobi OO, Cao M, Wang T, Cabrera R, Nelson DR, Liu C. OPA1 down-regulation is involved in sorafenib-induced apoptosis in hepatocellular carcinoma.　Lab Invest. 2013 Jan; 93 (1):8-19. (#Co-First author).       
  23. Tian, C*, Ambroz, R., Sun, L., Wang, Y., Ma, K., Chen, Q., Zhu, B. and Zheng, J*. (2012). Direct conversion of dermal fibroblasts into neural progenitor cells by a novel cocktail of defined factors. Current Molecular Medicine. 2012, 12(2):126-137. NIHMS 347018 (*Co-corresponding author). PMCID:  PMC3434966.
  24. Changhai Tian, Lijun Sun, Beibei Jia, Kangmu Ma, Norman Curthoys and Jialin Zheng. Mitochondrial glutaminase release contributes to glutamate-mediated neurotoxicity during human immunodeficiency virus -1 infection.  Journal of Neuroimmune Pharmacology. 2012, 7(3):619-28.
  25. Lixia Zhao, Yunlong Huang, Changhai Tian, Lynn Taylor, Norman Curthoys, Yi Wang, Hamilton Vernon, Jialin Zheng. Interferon-a Regulates Glutaminase 1 Promoter through STAT1 Phosphorylation: Relevance to HIV-1 Associated Neurocognitive Disorders. PLoS One. 2012; 7(3): e32995.
  26. Xin Huang, Changhai Tian, Miao Liu, Yongxiang wang, Aleksey V Tolmachev, Seema Sharma, Yu Fang, Kai Fu, Jialin Zheng, and Shi-Jian Ding. Quantitative Proteomic Analysis of Mouse Embryonic Fibroblasts and Induced Pluripotent Stem Cells Using 16O/18O labeling. J Proteome Res. 2012 Apr 6; 11(4):2091-102.
  27. Tian, CH and Zheng, J. Reprogrammed Astrocytes as a Potential Therapy for Neurodegenerative Disorders. Science. 2011 Dec 23; 334 (6063 Suppl): 53-54.
  28. Changhai Tian*, Yongxiang Wang, Lijun Sun, Kangmu Ma and Jialin Zheng*. Reprogrammed Mouse Astrocytes Retain a “Memory” of Tissue Origin and Possess More Tendencies for Neuronal Differentiation than Reprogrammed MEFs. Protein Cell. 2011, 2(2):128-40 (*Co-correspondence author)
  29. Huang X, Liu M, Nold MJ, Tian C, Fu K, Zheng J, Geromanos SJ, Ding SJ. Software for quantitative proteomic analysis using stable isotope labeling and data independent acquisition. Anal Chem. 2011, 15; 83(18):6971-9.
  30. Cui M, Huang Y, Tian C, Zhao Y, Zheng J. FOXO3a inhibits TNF-α- and IL-1β-induced astrocyte proliferation: Implication for reactive astrogliosis. Glia, 2011, 59(4):641-54.
  31. Changhai Tian, L. Charles Murrin and Jialin C. Zheng. Mitochondrial fragmentation is involved in methamphetamine-induced cell death in rat hippocampal neural progenitor cells. PLoS ONE. 2009; 4 (5): e5546.
  32. Nathan Erdmann, Changhai Tian, Yunlong Huang, Jianxing Zhao, Shelley Herek, Norman Curthoys and Jialin Zheng. In Vitro Glutaminase regulation and mechanisms of glutamate generation in HIV-1 infected macrophage. J Neurochem. 2009,109 (2):551-61.
  33. Changhai Tian, Nathan Erdmann, Jianxin Zhao, Zhijun Cao, Hui Peng, Takashi Tsukamoto and Jialin Zheng. HIV-infected macrophages mediate neuronal apoptosis through mitochondrial glutaminase. J Neurochem. 2008,105 (3):994-1005.
  34. Changhai, Tian, Ping Gao, Yanhua Zheng, Wen Yue, Xiaohui Wang, Haijing Jin, Quan Chen. Redox status of thioredoxin-1 (TRX1) determines the sensitivity of human liver carcinoma cells (HepG2) to arsenic trioxide-induced cell death. Cell Res. 2008, 18 (4):458-471.
  35. Chunlai Nie, Changhai Tian, Lixia Zhao, Patrice Xavier Petit, Maryam Mehrpour and Quan Chen. Cysteine 62 of bax is critical for its conformational activation and its pro-apoptotic activity in response to H2O2-induced apoptosis. J Biol Chem. 2008, 283(22):15359-15369.
  36. Hui Peng, Nicholas Whitney, Yumei Wu, Changhai Tian, Huanyu Dou and Jialin Zheng. HIV-1-Infected and/or Immune-Activated Macropahge-Secreted TNF-a Affects Human Fetal Cortical Neural Progenitor Cell Proliferation and Differentiation. Glia, 2008, 56(8): 903-916.
  37. Nicholas P. Whitney, Hui Peng, Nathan B. Erdmann, Changhai Tian, Daniel T. Monaghan and Jialin C. Zheng. Activation of calcium-permeable AMPA receptors containing Q/R-unedited GluR2 directs human neural progenitor cells differentiation to neurons. FASEB J. 2008, 22(8): 2888-900.
  38. Zheng Y, Yamaguchi H, Tian C, Lee MW, Tang H, Wang HG, Chen Q. Arsenic trioxide (As2O3) induces apoptosis through activation of Bax in hematopoietic cells. Oncogene. 2005, 24 (20): 3339-47.
  39. Zheng Y, Shi Y, Tian C, Jiang C, Jin H, Chen J, Almasan A, Tang H, Chen Q. Essential Role of the Voltage-dependent Anion Channel (VDAC) in Mitochondrial Permeability Transition Pore Opening and Cytochrome C Release Induced by Arsenic Trioxide. Oncogene. 2004, 23 (6): 1239-47.