Analytical Core
The major function and primary strength of the Analytical Core is provision of reliable technically robust panels of assays for environmental contaminants and markers of exposure or behavior that are of interest to UK-CARES researchers. Consistent with the three science themes of the Center, these include methods to support measurements of environmental chemical or biomarkers in the areas of water, air quality, and emerging threats.
Assays for Environmental Contaminants and Markers of Exposure
Click here to download a list of assays developed by the Analytical Core leaders:
- Environmental Contaminant Analytical Panels
- Biomarkers of Tobacco and Opiates
- Nutritional Biomarkers
Laboratories
Environmental Chemistry and Toxicology Laboratory and Nutrient Analysis Laboratory
Located in Agriculture Science Center North building, these facilities contain a suite of laboratories configured for analytical chemistry and molecular biology that includes instrument rooms, a clean preparation room, fume hood rooms, and wet laboratories; freezers including ultra-low temperature freezers with temperature monitoring and emergency power; and office space for staff containing workstation computers for data analysis and reporting. The core laboratories contain the following instrumentation:
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Agilent 7900 ICP-MS
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Wyatt Technologies Eclipse III Field Flow Fractionation System
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Postnova analytics S-1000 sedimentation field flow fractionator
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Agilent 1200 series HPLC
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Agilent 1200 series CE
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CETAC/TELEDYNE 210nm Laser Ablation System
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Agilent 5110SVDV ICP-OES
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Dionex ICS-3000 Ion Chromatograph
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Agilent 8890 GC-ECD
Equine Analytical Drug Testing Laboratory
Additional analysis will be conducted at the Equine Analytical Drug Testing (EADT) Laboratory. The Equine Laboratory occupies >1,500 sq. ft and underwent substantial custom renovations in order to be on the cutting edge of innovation for analytical science. The laboratory is well equipped with state-of-the-art instrumentation (Liquid Chromatography-Mass Spectrometers). In addition to instrumentation, the laboratory is equipped with SpeedDisk 48 place and 96 well positive pressure SPE manifolds, Zymark TurboVap – nitrogen evaporators, Sorvall centrifuges, Revco laboratory Refrigerators, Revco Laboratory Freezers, and a Revco -80°C Freezer. The laboratory currently has a license for the proteomic PEAKS™ and a license for the Compound Discoverer, both proteomic software programs. The equine laboratory contains the following instruments to support Center researchers:
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Thermo TSQ Altis Triple Quadrupole, Mass Spectrometer w/Vanquish UHPLC
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Thermo Orbitrap Exploris 480, Mass Spectrometer w/UltraMate 3000 NanoLC
Small Molecule Mass Spectrometry Facility (SMMF)
Located in the Biomedical and Biological Sciences Research Building, the SMMF contains a suite of laboratories configured for analytical chemistry/mass spectrometry that includes instrument rooms, a room housing air compressors to operate gas generators and solvent evaporators, ultra-low temperature freezers with temperature monitoring and emergency power, wet laboratory/bench space for sample preparation, and office space for staff with workstation computers for data analysis and reporting. The SMMF contains the following instrumentation:
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Agilent 7890 GC/ 5975 mass selective detector and electron capture detector
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Agilent 7890B Gas Chromatograph with Agilent 7000C triple quadrupole mass spectrometer
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Two Teledyne Tekmar Atomx combined soil/water autosampler and purge trap concentrators
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Two AB Sciex 4000 Q-Trap triple quadrupole linear ion trap/UHPLC mass spectrometer systems
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ABSciex 5600 Quadrupole time of flight/UHPLC mass spectrometer system
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Thermo Q-Exactive quadrupole orbitrap/UHPLC mass spectrometer system
Leadership
Staff: Shristi Shrestha, BS, Jianzhong Chen, PhD, and Yu Li, MS